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| Resources: Books
LC
Resources carries classic texts on HPLC and MS that are a must for
every laboratory. You can order these books from Amazon simply by
clicking the book cover images below. Used copies are sometimes available at
discounted rates.
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Introduction to Modern Liquid Chromatography,
3rd Ed
Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan
Snyder and Kirkland's Introduction to Modern Liquid Chromatography
has long represented the premier reference to HPLC. This Third Edition,
with John Dolan as added coauthor, addresses important improvements in
columns and equipment, as well as major advances in our understanding
of HPLC separation, our ability to solve problems that were troublesome
in the past, and the application of HPLC for new kinds of samples.
This carefully considered Third Edition maintains the strengths of the
previous edition while significantly modifying its organization in
light of recent research and experience. The text begins by introducing
the reader to HPLC, its use in relation to other modern separation
techniques, and its history, then leads into such specific topics as:
- The basis of HPLC separation and the general effects of different experimental conditions
- Equipment and detection
- The column—the "heart" of the HPLC system
- Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques
- Computer simulation, qualitative and quantitative analysis, and method validation and quality control
- The separation of large molecules, including both biological and synthetic polymers
- Chiral separations, preparative separations, and sample preparation
- Systematic development of HPLC separations—new to this edition
- Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms
Designed to fulfill the needs of the full range of HPLC users,
from novices to experts, Introduction to Modern Liquid Chromatography,
Third Edition offers the most up-to-date, comprehensive, and accessible
survey of HPLC methods and applications available.
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Practical HPLC
Method Development, 2nd Ed
Lloyd R. Snyder, Joseph L. Glajch, and Joseph J. Kirkland; 800 pp.
Thoroughly revised and expanded, this new edition of the best-selling
reference brings the reader completely up-to-date on the newest HPLC
methods. Since the publication of the first edition of this critically
acclaimed work, HPLC methods have evolved dramatically with
increasingly more complex sample types being analyzed. Written by the
preeminent authorities on HPLC, Practical HPLC Method Development
reflects the latest advances in the technology and applications of this
highly popular analytical method and provides both an exceptional
reference and practical introduction to the field of HPLC.
This title also incorporates updated discussions of fundamental systems
components and practical issues associated with HPLC that were
originally introduced in Snyder and Kirkland's classic work
Introduction to Modern Liquid Chromatography. Practical HPLC Method
Development represents an ideal instructional tutorial for scientists
and students who are just beginning to work with HPLC. The
practice-oriented coverage will benefit the experienced analyst as well
in developing better HPLC methods faster.
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Troubleshooting LC
Systems
John Dolan and Lloyd Snyder; 515 pp.
This book makes available the authors' wealth of experience from years
of troubleshooting real-world problems that cause crises and downtime
in almost every laboratory. The book opens by reviewing the fundamental
principles of HPLC operation, then moves on to a detailed description
of all the components of an LC system. Dolan and Snyder follow two main
themes: preventive maintenance (the best problems are those that never
occur) and a logical approach to diagnosis and identification of
problems that do occur. Both themes are implemented with a wide range
of specific recommendations, standard procedures, and troubleshooting
tables applicable to virtually every HPLC system in use. This is an
essential laboratory handbook: no one using an HPLC should be without
it.
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High-Performance
Gradient Elution: The Practical Application of the
Linear-Solvent-Strength Model
Lloyd Snyder and John Dolan; 496 pp.
This book takes the mystery out of the practice of gradient elution and
helps remove barriers to the practical application of this important
separation technique. It presents a systematic approach to the current
understanding of gradient elution, describing theory, methodology, and
applications across many of the fields that use liquid chromatography
as a primary analytical tool.
This up-to-date, practical, and comprehensive treatment of gradient
elution:
- Provides specific,
step-by-step recommendations for developing a gradient separation for
any sample
- Describes the best
approach for troubleshooting problems with gradient methods
- Guides the reader
on the equipment used for gradient elution
- Lists which
conditions should be varied first during method development, and
explains how to interpret scouting gradients
- Explains how to
avoid problems in transferring gradient methods
With a focus on the
use of linear solvent strength (LSS) theory for predicting gradient LC
behavior and separations by reversed-phase HPLC, High-Performance
Gradient Elution gives every chromatographer access to this useful
tool.
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Basic HPLC and CE
of Biomolecules
Robert Cunico, Karen Gooding, and Tim Wehr; 388 pp. (paperback)
Anyone currently using HPLC or CE for biomolecules or planning to
implement these techniques in the future will find this book
invaluable. It not only provides explanation and examples of the
primary chromatographic techniques for biomolecules, but it also
provides practical guidelines for their implementation and
optimization. The extensive bibliography provides a wealth of
background information.
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Modern HPLC for
Practicing Scientists
Michael Dong; 304 pp. (paperback)
An excellent introduction to HPLC. Covers all the basics clearly,
concisely and thoroughly.
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HPLC
Columns: Theory, Technology, and Practice
Uwe Neue; 416 pp.
An in-depth guide to
HPLC column technology High-performance liquid chromatography and its
derivative techniques have become the dominant analytical separation
tools in the pharmaceutical, chemical, and food industries;
environmental laboratories; and therapeutic drug monitoring. Although
the column is the heart of the HPLC instrument and essential to its
success, until now, no book has focused on the theory and practice of
column technology. HPLC Columns provides thorough, state-of-the-art
coverage of HPLC column technology for the practicing technician and
academician alike. Along with a comprehensive discussion of the
chemical and physical processes of the HPLC column, it includes
fundamental principles, separation mechanisms and available
technologies, column selection criteria, and special techniques.
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Validating Chromatographic Methods: A Practical Guide
David M. Bliesner; 291 pp. (hardcover)
This is as close to a "cookbook" for validation as you will find. Includes an extensive set of templates for valiation SOPs.
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Introduction
to Mass Spectrometry: Instrumentation, Applications, and Strategies for
Data Interpretation, 4th ed.
J. Throck Watson and O. David Sparkman; 862 pp.
Completely revised
and updated, this text provides an easy-to-read guide to the concept of
mass spectrometry and demonstrates its potential and limitations.
Written by internationally recognised experts and utilising "real life"
examples of analyses and applications, the book presents real cases of
qualitative and quantitative applications of mass spectrometry. Unlike
other mass spectrometry texts, this comprehensive reference provides
systematic descriptions of the various types of mass analysers and
ionisation, along with corresponding strategies for interpretation of
data. The book concludes with a comprehensive 3000 references.
This
multi-disciplined text covers the fundamentals as well as recent
advance in this topic, providing need-to-know information for
researchers in many disciplines including pharmaceutical, environmental
and biomedical analysis who are utilizing mass spectrometry
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