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Resources: Books

LC Resources carries classic texts on HPLC and MS that are a must for every laboratory. You can order these books from Amazon simply by clicking the book cover images below. Used copies are sometimes available at discounted rates.

Introduction to Modern Liquid Chromatography,
3rd Ed
Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan

Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.

This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:
  • The basis of HPLC separation and the general effects of different experimental conditions
  • Equipment and detection
  • The column—the "heart" of the HPLC system
  • Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques
  • Computer simulation, qualitative and quantitative analysis, and method validation and quality control
  • The separation of large molecules, including both biological and synthetic polymers
  • Chiral separations, preparative separations, and sample preparation
  • Systematic development of HPLC separations—new to this edition
  • Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms
Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Practical HPLC Method Development, 2nd Ed
Lloyd R. Snyder, Joseph L. Glajch, and Joseph J. Kirkland; 800 pp.

Thoroughly revised and expanded, this new edition of the best-selling reference brings the reader completely up-to-date on the newest HPLC methods. Since the publication of the first edition of this critically acclaimed work, HPLC methods have evolved dramatically with increasingly more complex sample types being analyzed. Written by the preeminent authorities on HPLC, Practical HPLC Method Development reflects the latest advances in the technology and applications of this highly popular analytical method and provides both an exceptional reference and practical introduction to the field of HPLC.

This title also incorporates updated discussions of fundamental systems components and practical issues associated with HPLC that were originally introduced in Snyder and Kirkland's classic work Introduction to Modern Liquid Chromatography. Practical HPLC Method Development represents an ideal instructional tutorial for scientists and students who are just beginning to work with HPLC. The practice-oriented coverage will benefit the experienced analyst as well in developing better HPLC methods faster.

Troubleshooting LC Systems
John Dolan and Lloyd Snyder; 515 pp.

This book makes available the authors' wealth of experience from years of troubleshooting real-world problems that cause crises and downtime in almost every laboratory. The book opens by reviewing the fundamental principles of HPLC operation, then moves on to a detailed description of all the components of an LC system. Dolan and Snyder follow two main themes: preventive maintenance (the best problems are those that never occur) and a logical approach to diagnosis and identification of problems that do occur. Both themes are implemented with a wide range of specific recommendations, standard procedures, and troubleshooting tables applicable to virtually every HPLC system in use. This is an essential laboratory handbook: no one using an HPLC should be without it.


High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model
Lloyd Snyder and John Dolan; 496 pp.

This book takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. It presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool.

This up-to-date, practical, and comprehensive treatment of gradient elution:
  • Provides specific, step-by-step recommendations for developing a gradient separation for any sample
  • Describes the best approach for troubleshooting problems with gradient methods
  • Guides the reader on the equipment used for gradient elution
  • Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients
  • Explains how to avoid problems in transferring gradient methods

With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.

Basic HPLC and CE of Biomolecules
Robert Cunico, Karen Gooding, and Tim Wehr; 388 pp. (paperback)

Anyone currently using HPLC or CE for biomolecules or planning to implement these techniques in the future will find this book invaluable. It not only provides explanation and examples of the primary chromatographic techniques for biomolecules, but it also provides practical guidelines for their implementation and optimization. The extensive bibliography provides a wealth of background information.

Modern HPLC for Practicing Scientists
Michael Dong; 304 pp. (paperback)

An excellent introduction to HPLC. Covers all the basics clearly, concisely and thoroughly.

HPLC Columns: Theory, Technology, and Practice
Uwe Neue; 416 pp.

An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques.

Validating Chromatographic Methods: A Practical Guide
David M. Bliesner; 291 pp. (hardcover)

This is as close to a "cookbook" for validation as you will find. Includes an extensive set of templates for valiation SOPs.

Introduction to Mass Spectrometry: Instrumentation, Applications, and Strategies for Data Interpretation, 4th ed.
J. Throck Watson and O. David Sparkman; 862 pp.

Completely revised and updated, this text provides an easy-to-read guide to the concept of mass spectrometry and demonstrates its potential and limitations. Written by internationally recognised experts and utilising "real life" examples of analyses and applications, the book presents real cases of qualitative and quantitative applications of mass spectrometry. Unlike other mass spectrometry texts, this comprehensive reference provides systematic descriptions of the various types of mass analysers and ionisation, along with corresponding strategies for interpretation of data. The book concludes with a comprehensive 3000 references.

This multi-disciplined text covers the fundamentals as well as recent advance in this topic, providing need-to-know information for researchers in many disciplines including pharmaceutical, environmental and biomedical analysis who are utilizing mass spectrometry


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