Making LC Methods Work

2018 Schedule

Making LC Methods Work is presented Live on the Web in three 2.5-hour sessions from 8:00 - 10:30 am (Pacific Daylight Time).

September 17, 19, & 21

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Live on the Web: Making LC Methods Work

Who should take this course?

If you use HPLC or UHPLC methods and want a thorough grounding in what to do when problems arise, then , "Making LC Methods work" is the course for you. It's designed for chemists and biochemists who use LC as a regular part of their jobs, but technicians with some LC experience will also find the course valuable. No previous formal LC training is assumed, but participants should have at least 6 months of "hands-on" experience with liquid chromatography.

What does it cover?

"Making LC Methods Work" focuses on reversed-phase chromatography, and assumes that you are starting with an existing, validated (or at least, "known to work") method. We'll cover things like:

Major causes of failure to pass system suitability tests
How to tell whether problems are due to chemistry or hardware
A systematic strategy to identify problem causes efficiently
How to maximize column life
Special techniques such as gradient elution, quantitation, and sample pretreatment
The most effective, timesaving, money saving approaches to preventing common method failures.

. . . And much, much more

How much does it cost ?

The standard "Live on the Web" course fee is $947/person. This includes the course and a download of the course notes. Payment via credit card (MC, VISA, or AMEX only).

Additional registrants from the same company are 30% off the regular price. Call us at (925) 297-5374 to arrange multiple-registrant pricing.

What do I need in order to take the course?

A personal computer or tablet (Windows, Mac,iOS,or Android)
A high-speed (broadband) internet connection
A phone connection with headset or speakerphone

Here's how it works:

After you register we'll e-mail your course log-in information and password.
Download and print a hard copy of the course handout (approximately 200 pages).
About five minutes before the start of the course, log in to www.gotomeeting.com to download the gotomeeting client and check in for the course. Dial the phone number* for the conference call connection that you were given in your confirmation e-mail.
The course runs a total of six hours in three 2.5-hour sessions .

*Standard long-distance phone rates will apply (approximately $10.00).

What if I can't make it?

All sessions are recorded and will be available for review for a month after the end of the course; if you miss a session, you can catch up at your convenience. If you must cancel, please call at least 48 hours prior to the start of the course in order to receive a refund. No refund will be made for cancellation within 48 hours of the course.

About the instructors
The course was developed by Lloyd Snyder, John Dolan, and Tom Jupille. These sessions will be presented by Tom Jupille.

Registration cost: $947 for a single person. Additional registrants from the same lab are 30% off the regular price; payment via credit card (MC, VISA, or AMEX only).

What topics are covered?

The course is presented in three 2.5-hour sessions "live on the web". This course is interactive, so the exact timing will vary somewhat depending on participants' interests and questions. A typical schedule looks like this:

Section 1. How do I know it's not working?

System suitability
The troubleshooting process
Adjustment vs. modification

Section 2. LC by the numbers

Direct measurements on the chromatogram
Calculated parameters
Retention and selectivity
Efficiency
Peak symmetry
Resolution

Section 3. Gradients

When / why use gradients
Controlling gradient separations
Dwell volume
Key differences between gradient and isocratic separations

Section 4. Retention / selectivity issues (reversed-phase)

Mobile phase strength
Temperature
pH
Ion pair chromatography
Maximizing column lifetime
Column substitution
Ambiguous documentation

Section 5. Peak width / shape issues

Extra-column volume
Tubing
Fittings
Detectors
Inlet flow anomalies
Stationary phase chemistry
Thermal equilibration
Overload
Late-eluting peaks

Section 6. Quantitation

Integration
Noise and drift
Gradient baseline issues
Calibration
Internal vs. external standardization
LOD / LOQ
Injector issues

Section 7. HPLC / UHPLC Method Transfer

Scaling isocratic separations
Scaling gradient separations
Possible surprises

Section 8. Diagnostic Tests

Pump & detector
Gradient proportioning
Autosampler

Section 9. Troubleshooting Checklist

No peaks
Missing peaks
Extra peaks
Wrong retention time
Poor efficiency or peak shape
Poor resolution
Poor precision
Baseline noise or drift
Pressure problems
Leaks
Column lifetime