Who should take this course?
If you've ever wished that HPLC method development were more logical and methodical, this is the course for you. Attendees should have at least one year of HPLC experience in the laboratory and at least some involvement in developing new methods or troubleshooting older ones. This course is for chromatographers who want to develop better methods faster and less expensively.

What does it cover?

In this two-day workshop, you will learn a powerful new approach to systematic method development. Learn the modern approach to method development:

• Define your objectives clearly in chromatographic terms such as resolution, run time, specificity, accuracy, precision, limit of detection, etc.
• Identify the key variables that affect retention, selectivity, and efficiency
• Select initial conditions that maximize the chances of quickly developing a workable method.
• Set up a systematic strategy for efficiently evaluating the effects of the key variables on your separation
• Take advantage of recent improvements in column technology to assist you in your method development efforts
• Learn to develop robust methods that have superior separation repeatability
• Design system suitability checks and validation into your method
• Understand the factors that control precision, accuracy, and robustness.

This course makes extensive use of chromatography simulation software as a teaching tool. Course attendees work in teams to develop methods using the principles discussed during the lectures. You get to apply your new knowledge to your own and other students' actual data during in-class problem-solving sessions. The principles used in computer modeling are fully applicable to separations in your lab. You will learn how to do the best possible chromatography. Save hours of frustration and improve your results time after time.

How much does it cost ?

The standard course fee is $1297/person. This includes the course, notes, continental breakfasts and lunches. "Live on the Web" course fee is $997/person. This includes the course and a download of the course notes.

Additional registrants from the same company are half-price. Call us at (800) 379-5221 to arrange multiple-registrant pricing

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Schedule for this course

Course Outline

Day 1

Registration/continental breakfast
Introduction
Introduction HPLC Method Development
Review of Separation Basics
Workshop: Varying %B and Column Conditions
Lunch
New Column Developments
Reversed-Phase HPLC for Neutral Samples
Workshop: Effects of Solvent Type on Separation
Workshop: Mixing Solvents
Reversed-Phase HPLC of Ionic Samples
Workshop: Optimizing pH and %B for a Mixture of Acids and Bases

Day 2

Continental breakfast
Ion-Pair HPLC
Workshop: Ion-Pair Chromatography
Gradient Elution
Workshop: Gradient Elution
Lunch
Bonus Material (per attendees interests):

Quality Issues

Normal Phase and HILIC

Ion Exchange / Chiral / Prep

Sample Prep

LC/MS Issues

Discussion of Student Problems